� it the levels of iep, zn and changed after dialysis, due amitriptyline and ergotamine to the removal of molecules that were poorly linked mainly free peg at the outer part of the surface, allowing accessibility to the inner adjacent part of the shell water shell fig accessible layer to counter ions characterized by its thickness x and its dipolar charge density amitriptyline and ergotamine zn nm lnc presented the bestorganized and the accessible part of the shell, compared with other sizes of lnc, before and after dialysis amitriptyline and ergotamine lecithin was found to be present in the inner part of the polyelectrolyte layer and was found to play a role in the amitriptyline and ergotamine disorganization of the outer part dialyzing lnc formulated with lecithin led to stable and well structured nanocapsules, ready for an in vivo use as a drug delivery system evaluation of complement system activation generally, after intravenous administration, nanoparticles np are rapidly removed from the blood stream because they are recognized by cells of the mps such as kiipffer cells in the liver, or spleen and bonemarrow macrophages however, a brush of peg chains grafted amitriptyline and ergotamine on the surface is known to decrease the recognition of nanoparticles by the immune system after intravenous administration one has demonstrated that a strong correlation prevails between the complement activation and the stealthy properties of lnc therefore, these properties were evaluated by measuring the degree of complement activation [ch technique and crossed immunoelectrophoresis c cleavage] and the level of macrophage uptake, in relation to the amitriptyline and ergotamine organization of peg chains, according to the electrokinetic properties of the lnc side effects women lipitor surface these experiments were performed on , and nm lnc before and after dialysis the ch technique is presented in fig nanoparticles are dispersed in human serum with sensitized erythrocytes after amitriptyline and ergotamine incubation, lysis is evaluated by a classical spectrophotometric method amitriptyline and ergotamine the measured absorbance is related to the consumption of complement proteins by particles the main conclusions are that whatever the in vitro test, all lnc were not recognized by the non specific components of the immune system it was probably due amitriptyline and ergotamine to the strong density of peg chains at their surface furthermore, dialysis maintains a sufficiently high density of peg and had no incidence on the complement consumption pharmacokinetic studies and biodistribution at first, the biodistribution of radiolabeled nanocapsules amitriptyline and ergotamine was studied by scintigraphy and � counting, after intravenous administration in rat whereby the mtcoxine was incorporated in the lipid core and i eryc 250mg labelled the shell of the nanocapsules dynamic scintigraphic acquisition was carried out hrs after administration and � activity in blood and tissues was followed for more than hrs see fig an early halfdisappearance time of about � min was found for amitriptyline and ergotamine i and � min for mtc these ranges of residence times were interesting for specific �a st active wcd�s vcub nnnnil scrum cdds vr i ?