when looking at data obtained from studies with living mammalian cells, it appears reasonable to assume that dequalinium molecules could be pulled into the mitochondrial matrix in response to the high mitochondrial membrane potential metformin sleep disturbance as demonstrated in , which in turn might lead to the destabilization of the dqasomepdna complex however, the first detailed study, which demonstrated the selective dna release from dqaplexes, was performed using membrane mimicking liposomes fig metformin sleep disturbance as a model for the intracellular release of dna from dqasomes, the capacity of anionic liposomes to displace the dna metformin sleep disturbance from its cationic carrier was studied the association of dna metformin sleep disturbance with the cationic carrier was assessed by employing sybr green i the fluorescence signal of metformin sleep disturbance this dye is greatly enhanced when bound to dna nonbinding results metformin sleep disturbance in loss of fluorescence it can be clearly seen that in the vicinity of a charge ratio, dqasomes do not release any dna in the metformin sleep disturbance presence of cytoplasmic membrane mimicking liposomes dqasomes s � � metformin sleep disturbance o � u a � � � cpm imm ��� anionic liposomes i � imitrex behavior � time [sec] fig effect of anionic liposomes on dna release from dqasomepdna complexes dna was metformin sleep disturbance preincubated with sybr until stabilization of the signal, followed by metformin sleep disturbance adding indicated by arrow the minimal amount of dqasomes necessary to decrease the signal to metformin sleep disturbance background level anionic liposomes were then injected arrow at an anionic to cationic charge ratio as shown the displacement of dna from its carrier is metformin sleep disturbance indicated by the increase of the fluorescence signal cpm, cytoplasma membrane like liposomes imm, inner mitochondrial membrane like liposomes omm, outer mitochondrial membrane like liposomes cpm, not even at a fold excess of anionic charge however, with a similar charge excess of anionic liposomes to cationic metformin sleep disturbance dqasomes, and respectively, inner and outer mitochondrial membrane mimicking liposomes metformin sleep disturbance imm and omm, respectively are metformin sleep disturbance able to displace up to of the dna from its dqasomal carrier in agreement with these data, it was found that for the complete liberation of dna from dna dqasome complex, a fourfold excess of dicetylphosphate and an eightfold excess of phosphatidylserine, home made lithium battery respectively, are necessary metformin sleep disturbance the finding that cpm liposomes, at an anionic to cationic charge ratio of , displace up metformin sleep disturbance to of the dna from metformin sleep disturbance lipofectin, which was used as a control, do not liberate any dna from dqasomes even at a slight excess of anionic charge, leads to the conclusion that besides the charge ratio, other factors may play an important role in the mechanism of dna release from lipiddna metformin sleep disturbance complexes this conclusion is being further supported by xu and szokas observation that ionic water soluble molecules such as atp, trna, dna, polyglutamic acid, spermidine and histone do not displace dna from the cationic lipiddna complex, even at a fold metformin sleep disturbance charge excess � in their model for the postendocytotic release of dna from cationic carriers, they assume the formation of a charge neutral ion pair metformin sleep disturbance between cationic and anionic lipid, which ultimately results in the displacement of the dna from the cationic lipid and the metformin sleep disturbance release of dna into cytoplasm liposomedna liposome ?